Skip to content Immunology and Infection. A pipette tip is gently inserted about 5 mm deep into the vaginal lumen. No data have been published about the possibility of achieving candidal vaginitis in these animals. In uninfected mice, mainly epithelial and, rarely, immune cells, were detected. A detailed description of the model has been reported elsewhere A drop of this material was placed between a glass slide and coverslip and observed fresh by ordinary optical microscopy under 10X and 40X objectives. Similar results for vaginal fungal titers and DTH were observed when progesterone was given at threefold-higher concentrations 1.
Effects of Reproductive Hormones on Experimental Vaginal Candidiasis
In our experimental conditions CD4 positive cells appeared the main source of IL, with maximum intracellular expression between days 8 and 21 Fig. Both rats and mice must be placed under stable pseudoestrus conditions to establish experimental vaginal infection. Th17 cells in immunity to Candida albicans. Conclusions These results imply that IL and Th17, along with innate antimicrobial factors, have a role in the immune response to vaginal candidiasis. To determine if the lack of effect of progesterone on vaginal fungal burden in the presence of estrogen was due to the high concentrations of estrogen used, a similar experiment was performed with a fold-lower concentration of estrogen 0. Progesterone had no effect on the partial protection against the second infection Fig.
Experimental Models of Vaginal Candidiasis and Their Relevance to Human Candidiasis
All efforts were made to minimize suffering during experiments. It is generally accepted that cell-mediated immunity CMI by T cells and cytokines specifically a Th1-type response is the dominant host defense mechanism against C. This article has been cited by other articles in PMC. In mice, a pioneering study was conducted by Cutler's research group showing that animals fed with antibiotic-containing water were stably colonized by the fungus This study extended this finding to include the fact that estrogen treatment of mice significantly reduced epithelial-cell-mediated anti- Candida activity. Overall, among the many putative virulence factors of C. Comparison of ketoconazole, Bay N, and Bay L in the treatment of experimental vaginal candidiasis.
Concerning the hypothesis that the presence of a cornified epithelium predisposes to VVC, our results also demonstrated the presence of abundant cornified cells in PCG, as previously described by other authors Smith et al. Striking differences from the rat model regarding mechanisms of immune responses and inflammation have been reported in most of the studies mentioned above. History and update on host defense against vaginal candidiasis. Serial dilutions of up to 12 lavage samples one full horizontal row can be performed simultaneously using a channel pipette. This technique allows continuous, non-invasive monitoring of the spatial and temporal progression of vaginal infection in live mice and has proved useful to evaluate vaccinal and immunotherapeutic approaches to the treatment of vaginal candidiasis .